Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sharma, Ruchi
- Efficient molecular method/s for detection of Bt brinjal Event 142
Abstract Views :173 |
PDF Views:96
Authors
Affiliations
1 Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, IN
1 Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110 012, IN
Source
Current Science, Vol 122, No 4 (2022), Pagination: 464-468Abstract
Fruit and shoot borer (FSB)-resistant Bt brinjal Event 142 with cry1Fa1 gene has been approved for biosafety research level (BRL)-II field trials by the Genetic Engineering Appraisal Committee (GEAC), the competent authority in India. For regulatory compliance, systematic detection of genetically modified (GM) crops would facilitate monitoring for the presence of a GM event in the supply chain. Polymerase chain reaction (PCR) and real-time PCR are widely used for GM detection globally. Qualitative PCR and real-time PCR assays targeting cry1Fa1 transgene were developed and validated with acceptable specificity and sensitivity up to 0.01%. A multiplex (hexaplex) PCR-based screening assay simultaneously detecting five transgenic elements and an endogenous gene was developed for Event 142. Construct-specific PCR reported herein could be employed for more specific detection. These detection assays would facilitate efficient regulatory compliance by checking unapproved Bt brinjal event in the supply chain and also address the concerns of the consumers to an extent.Keywords
Bt brinjal, detection assays, field trials, genetically modified crops, polymerase chain reaction.Full Text
References
- International Service for the Acquisition of Agri-biotech Applications (ISAAA), Global status of commercialized biotech/GM crops in 2019: Biotech crops drive socio economic development and sustainable environment in the new frontier. ISAAA Brief No. 55, ISAAA, New York, USA, 2019.
- http://www.isaaa.org/gmapprovaldatabase/cropslist/default.asp (accessed on 24 October 2021).
- Choudhary, B. and Gaur, K., The development and regulation of Bt brinjal in India (Eggplant/Aubergine). ISAAA Brief No. 38, ISAAA, Ithaca, NY, USA, 2009.
- Kumar, P. A. et al., Insect-resistant transgenic brinjal plants. Mol. Breed., 1998, 4(1), 33–37.
- Pal, J. K., Singh, M., Rai, M., Satpathy, S., Singh, D. V. and Kumar, S., Development and bioassay of Cry1Ac-transgenic eggplant (Solanum melongena L.) resistant to shoot and fruit borer. J. Hortic. Sci. Biotech., 2009, 84(4), 434–438.
- Rai, N. P., Rai, G. K., Kumar, S., Kumari, N. and Singh, M., Shoot and fruit borer resistant transgenic eggplant (Solanum melongena L.) expressing cry1Aa3 gene: development and bioassay. Crop Prot., 2013, 53, 37–45.
- Randhawa, G. J., Singh, M. and Sood, P., DNA-based methods for detection of genetically modified events in food and supply chain. Curr. Sci., 2016, 110(6), 1000–1009.
- Randhawa, G. J., Sharma, R. and Singh, M., Qualitative and event-specific real-time PCR detection methods for Bt brinjal event EE-1. J. AOAC Int., 2012, 95(6), 1733–1739.
- Kuwardadra, S. I., Bhatt, K. C., Paliwal, A., Sood, P., Malav, P. K., Sodhi, K. K. and Randhawa, G. J., Monitoring adventitious presence of transgenes in brinjal (Solanum melongena L.) collections from the regions in India bordering Bangladesh: a case report. Genet. Resour. Crop Evol., 2020, 67, 1181–1192.
- Kuwardadra, S. I., Pandey, C. D., Paliwal, A. and Randhawa, G. J., Screening brinjal accessions conserved in the National Genebank collected from North Eastern states of India for adventitious
- presence of EE1 event. Indian J. Agric. Sci., 2020, 90(8), 1603–1610.
- https://geacindia.gov.in/Uploads/MoMPublished/MoMPublishedOn20200615165821.pdf (accessed on 24 October 2021).
- IARI, Synthetic gene encoding Cry1Fa1 delta-endotoxin of Bacillus thuringiensis. India Patent No. 242768, 2010.
- Randhawa, G. J., Chhabra, R. and Singh, M., Decaplex and realtime PCR based detection of MON531 and MON15985 Bt cotton events. J. Agric. Food Chem., 2010, 58(18), 9875–9881.
- Chhabra, R., Randhawa, G. J., Bhoge, R. K. and Singh, M., Qualitative and quantitative PCR-based detection methods for authorized genetically modified cotton events in India. J. AOAC Int., 2014, 97(5), 1299–1309.
- Randhawa, G. J., Singh, M., Chhabra, R. and Sharma, R., Qualitative and quantitative molecular testing methodologies and traceability systems for Bt crops commercialised or under field trials in India. Food Anal. Methods, 2010, 3(4), 295–303.
- Randhawa, G. J. and Singh, M., Multiplex, construct-specific and real-time PCR-based analytical methods for Bt rice with cry1Ac gene. J. AOAC Int., 2012, 95(1), 186–194.
- Randhawa, G. J., Chhabra, R. and Singh, M., Molecular characterization of Bt cauliflower with multiplex PCR and validation of endogenous reference gene in Brassicaceae family. Curr. Sci., 2008, 95(12), 1729–1731.
- Thion, L., Vossen, C., Couderc, B., Erard, M. and Clemençon, B., Detection of genetically modified organisms in food by DNA extraction and PCR amplification. Biochem. Mol. Biol. Educ., 2002, 30(1), 51–55.
- Chaouachi, M., Malki, R. L., Berard, A., Romaniuk, M., Laval, V., Brunel, D. and Bertheau, Y., Development of a real-time PCR method for the differential detection and quantification of four Solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum). J. Agric. Food Chem., 2008, 56(6), 1818–1828.